Tropilaelaps mercedesae is without doubt one of the most problematic honey bee parasites and has change into extra threatening to the beekeeping business. Tropilaelaps can simply parasitize immature honey bees (larvae and pupae) and have each deadly and sublethal results on the person employee bees. Our examine for the primary time experimentally assessed the consequences of T. mercedesae on olfactory studying, flight means, homing means in addition to transcriptional changes in parasitized grownup honey bees.
T. mercedesae infestation had adverse impacts on olfactory related perform, flight means, and homing charge. The quantity of the mushroom physique considerably elevated in infested honey bees, which can be correlated to the decrease sucrose responsiveness in addition to decrease studying means in the infested bees.
The gene expression concerned in immune programs and carbohydrate transport and metabolism have been considerably completely different between infested bees and non-infested bees. Moreover, genes perform in cell adhesion play an important function in olfactory sensory in honey bees. Our findings present a complete understanding of European honey bees in response to T. mercedesae infestation, and might be used to additional examine the complicated molecular mechanisms in honey bees beneath parasitic stress.
Loss of inner-envelope Ok+/H+ exchangers impairs plastid rRNA maturation and gene expression
The inner-envelope Ok+ EFFLUX ANTIPORTERS (KEA) 1 and 2 are essential for chloroplast improvement, ion homeostasis, and photosynthesis. However, the mechanisms by which changes in ion flux throughout the envelope have an effect on organelle biogenesis remained elusive. Chloroplast improvement requires intricate coordination between the nuclear genome and the plastome. Many mutants compromised in plastid gene expression (PGE) show a virescent phenotype, i.e. delayed greening.
The phenotypic look of Arabidopsis thaliana kea1 kea2 double mutants fulfills this criterion, but a hyperlink to PGE has not been explored. Here, we present {that a} simultaneous lack of KEA1 and KEA2 outcomes in maturation defects of the plastid ribosomal RNAs. This could also be brought on by secondary construction changes of rRNA transcripts and concomitant lowered binding of RNA-processing proteins, which we documented in the presence of skewed ion homeostasis in kea1 kea2.
Consequently, protein synthesis and steady-state ranges of plastome-encoded proteins stay low in mutants. Disturbance in PGE and different indicators of plastid malfunction activate GUN1-dependent retrograde signaling in kea1 kea2, ensuing in a dramatic downregulation of GOLDEN2-LIKE transcription components to halt expression of photosynthesis-associated nuclear-encoded genes (PhANGs). PhANG suppression delays the event of absolutely photosynthesizing kea1 kea2 chloroplasts, in all probability to keep away from progressing photo-oxidative harm. Overall, our outcomes reveal that KEA1/KEA2 perform impacts plastid improvement by way of results on RNA-metabolism and PGE.
Effects of Tris (2-chloroethyl) phosphate (TCEP) on survival, development, histological changes and gene expressions in juvenile yellow catfish Pelteobagrus fulvidraco
Tris (2-chloroethyl) phosphate (TCEP) is an rising aquatic environmental pollutant. In the current examine, juvenile yellow catfish (Pelteobagrus fulvidraco) have been uncovered to environmentally related concentrations of TCEP for 30 days. The outcomes confirmed that TCEP publicity decreased the survival charge (100 μg/L), physique weight (10 and 100 μg/L) and particular development charge (10 and 100 μg/L) of juvenile yellow catfish.
Exposure to TCEP resulted in pronounced damages of gill buildings. Gene transcription evaluation confirmed that the antioxidant capability of the liver and gills was affected; CYP1A1 may contribute to section I metabolism of TCEP in the liver slightly than CYP1B1; TCEP stress may improve the demand of ion transport in fish gill; TCEP may stimulate the immune response and may induce apoptosis by way of a p53-Bax pathway and caspase-dependent pathway in gills. Collectively, these findings present new insights into the poisonous results of TCEP on fish.
Knockdown of DNA methyltransferase 1 reduces DNA methylation and alters expression patterns of cardiac genes in embryonic cardiomyocytes
We beforehand discovered that DNA methyltransferase 3a (DNMT3a) performs an essential function in regulating embryonic cardiomyocyte gene expression, morphology, and perform. In this examine, we investigated the function of essentially the most plentiful DNMT in mammalian cells, DNMT1, in these processes. It is understood that DNMT1 is crucial for embryonic improvement, throughout which it’s concerned in regulating cardiomyocyte DNA methylation and gene expression.
We used siRNA to knockdown DNMT1 expression in major cultures of mouse embryonic cardiomyocytes. Immunofluorescence staining and multi-electrode array have been respectively utilized to judge cardiomyocyte development and electrophysiology. RNA-sequencing (RNA-Seq) and multiplex bisulfite sequencing have been respectively carried out to look at gene expression and promoter methylation. At 72 hours post-transfection, discount of DNMT1 expression decreased the quantity and elevated the scale of embryonic cardiomyocytes.
Beat frequency and the amplitude of area motion potentials have been decreased by DNMT1 siRNA. RNA-Seq evaluation recognized 801 upregulated genes and 494 downregulated genes in the DNMT1 knock-down cells when in comparison with controls. Pathway evaluation of the differentially expressed genes revealed pathways that have been related to cell demise and survival, cell morphology, cardiac perform, and cardiac illness. Alternative splicing evaluation recognized 929 differentially expressed exons, together with 583 up-regulated exons and 308 down-regulated exons.
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 418 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 575 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 429 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 591 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-p-48Tests | Reddot Biotech | 48 Tests | EUR 494 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-p-96Tests | Reddot Biotech | 96 Tests | EUR 684 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 450 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 622 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Rb-48Tests | Reddot Biotech | 48 Tests | EUR 450 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RD-BMP4-Rb-96Tests | Reddot Biotech | 96 Tests | EUR 622 |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-b-48T | DL Develop | 48T | EUR 493 |
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Description: A sandwich quantitative ELISA assay kit for detection of Bovine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-b-96T | DL Develop | 96T | EUR 641 |
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Description: A sandwich quantitative ELISA assay kit for detection of Bovine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-c-48T | DL Develop | 48T | EUR 474 |
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Description: A sandwich quantitative ELISA assay kit for detection of Canine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma or other biological fluids. |
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Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-c-96T | DL Develop | 96T | EUR 614 |
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Description: A sandwich quantitative ELISA assay kit for detection of Canine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma or other biological fluids. |
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Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Ch-48T | DL Develop | 48T | EUR 454 |
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Description: A sandwich quantitative ELISA assay kit for detection of Chicken Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Ch-96T | DL Develop | 96T | EUR 587 |
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Description: A sandwich quantitative ELISA assay kit for detection of Chicken Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-g-48T | DL Develop | 48T | EUR 503 |
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Description: A sandwich quantitative ELISA assay kit for detection of Goat Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-g-96T | DL Develop | 96T | EUR 654 |
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Description: A sandwich quantitative ELISA assay kit for detection of Goat Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Hu-48T | DL Develop | 48T | EUR 425 |
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Description: A sandwich quantitative ELISA assay kit for detection of Human Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Hu-96T | DL Develop | 96T | EUR 548 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Mu-48T | DL Develop | 48T | EUR 435 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Mu-96T | DL Develop | 96T | EUR 561 |
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Description: A sandwich quantitative ELISA assay kit for detection of Mouse Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-p-48T | DL Develop | 48T | EUR 493 |
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Description: A sandwich quantitative ELISA assay kit for detection of Porcine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-p-96T | DL Develop | 96T | EUR 641 |
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Description: A sandwich quantitative ELISA assay kit for detection of Porcine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Ra-48T | DL Develop | 48T | EUR 454 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Ra-96T | DL Develop | 96T | EUR 587 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rat Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
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Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Rb-48T | DL Develop | 48T | EUR 454 |
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Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| DLR-BMP4-Rb-96T | DL Develop | 96T | EUR 587 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
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Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-b-48Tests | Reddot Biotech | 48 Tests | EUR 516 |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-b-96Tests | Reddot Biotech | 96 Tests | EUR 716 |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-c-48Tests | Reddot Biotech | 48 Tests | EUR 493 |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-c-96Tests | Reddot Biotech | 96 Tests | EUR 683 |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Ch-48Tests | Reddot Biotech | 48 Tests | EUR 470 |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Ch-96Tests | Reddot Biotech | 96 Tests | EUR 651 |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-g-48Tests | Reddot Biotech | 48 Tests | EUR 528 |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-g-96Tests | Reddot Biotech | 96 Tests | EUR 733 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Hu-48Tests | Reddot Biotech | 48 Tests | EUR 436 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Hu-96Tests | Reddot Biotech | 96 Tests | EUR 601 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Mu-48Tests | Reddot Biotech | 48 Tests | EUR 447 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Mu-96Tests | Reddot Biotech | 96 Tests | EUR 618 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-p-48Tests | Reddot Biotech | 48 Tests | EUR 516 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-p-96Tests | Reddot Biotech | 96 Tests | EUR 716 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Ra-48Tests | Reddot Biotech | 48 Tests | EUR 470 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Ra-96Tests | Reddot Biotech | 96 Tests | EUR 651 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Rb-48Tests | Reddot Biotech | 48 Tests | EUR 470 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
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| RDR-BMP4-Rb-96Tests | Reddot Biotech | 96 Tests | EUR 651 |
HEK-293T Telomerase Over-Expressing Cell Pellet |
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| abx069991-1Pellet | Abbexa | 1 Pellet | EUR 398 |
Beaucage reagent |
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| HY-100951 | MedChemExpress | 10mM/1mL | EUR 126 |
BOP reagent |
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| 5-02141 | CHI Scientific | 25g | Ask for price |
BOP reagent |
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| 5-02142 | CHI Scientific | 100g | Ask for price |
Bradford reagent |
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| BDE641 | Bio Basic | 100ml | EUR 61.01 |
BOP reagent |
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| A7015-100000 | ApexBio | 100 g | EUR 200 |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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BOP reagent |
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| A7015-25000 | ApexBio | 25 g | EUR 113 |
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Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
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Bluing Reagent |
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| BRT030 | ScyTek Laboratories | 30 ml | EUR 60 |
Bluing Reagent |
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| BRT125 | ScyTek Laboratories | 125 ml | EUR 63 |
Bluing Reagent |
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| BRT3800 | ScyTek Laboratories | 1 Gal. | EUR 184 |
Bluing Reagent |
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| BRT500 | ScyTek Laboratories | 500 ml | EUR 76 |
Bluing Reagent |
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| BRT999 | ScyTek Laboratories | 1000 ml | EUR 88 |
Chymase reagent |
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| 30C-CP1129 | Fitzgerald | 5 units | EUR 2185 |
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Description: Purified native Human Chymase reagent |
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Traut's Reagent |
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| 2330-1000 | Biovision | EUR 349 | |
Traut's Reagent |
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| 2330-500 | Biovision | EUR 207 | |
MTS Reagent |
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| 2808-1000 | Biovision | EUR 990 | |
MTS Reagent |
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| 2808-250 | Biovision | EUR 365 | |
MTT Reagent |
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| 2809-1G | Biovision | EUR 180 | |
MTT Reagent |
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| 2809-5G | Biovision | EUR 544 | |
BMP4 Antibody |
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| 1-CSB-PA212919 | Cusabio |
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|
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Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:30-1:150 |
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BMP4 Antibody |
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| 1-CSB-PA002740GA01HU | Cusabio |
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|
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Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
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BMP4 Antibody |
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| 1-CSB-PA05799A0Rb | Cusabio |
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|
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Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat, Zebrafish. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000 |
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BMP4 Antibody |
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| 1-CSB-PA763132 | Cusabio |
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|
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Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100 |
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BMP4 Antibody |
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| 35652-100ul | SAB | 100ul | EUR 252 |
BMP4 siRNA |
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| 20-abx909174 | Abbexa |
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BMP4 siRNA |
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| 20-abx909175 | Abbexa |
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BMP4 antibody |
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| 38256-100ul | SAB | 100ul | EUR 252 |
BMP4 Antibody |
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| 43852-100ul | SAB | 100ul | EUR 252 |
BMP4 Antibody |
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| ABD6461 | Lifescience Market | 100 ug | EUR 438 |
BMP4 Antibody |
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| ABF5175 | Lifescience Market | 100 ug | EUR 438 |
BMP4 Antibody |
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| DF6461 | Affbiotech | 200ul | EUR 304 |
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Description: BMP4 Antibody detects endogenous levels of total BMP4. |
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BMP4 Antibody |
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| BF0696 | Affbiotech | 200ul | EUR 376 |
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Description: BMP4 antibody detects endogenous levels of total BMP4. |
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anti-BMP4 |
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| LF-PA0093 | Abfrontier | 100 ul | EUR 334 |
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Description: Rabbit polyclonal to BMP4 |
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BMP4 antibody |
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| 10R-B119a | Fitzgerald | 100 ug | EUR 792 |
|
Description: Mouse monoclonal BMP4 antibody |
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BMP4 antibody |
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| 10R-B119B | Fitzgerald | 500 ug | EUR 273 |
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Description: Mouse monoclonal BMP4 antibody |
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BMP4 antibody |
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| 10R-2148 | Fitzgerald | 100 ul | EUR 403 |
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Description: Mouse monoclonal BMP4 antibody |
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BMP4 antibody |
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| 10R-3460 | Fitzgerald | 100 ul | EUR 691 |
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Description: Mouse monoclonal BMP4 antibody |
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BMP4 antibody |
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| 70R-16011 | Fitzgerald | 50 ul | EUR 435 |
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Description: Rabbit polyclonal BMP4 antibody |
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BMP4 Antibody |
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| AF5175 | Affbiotech | 200ul | EUR 304 |
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Description: BMP4 Antibody detects endogenous levels of total BMP4. |
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BMP4 antibody |
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| 20R-2936 | Fitzgerald | 100 ul | EUR 393 |
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Description: Rabbit polyclonal BMP4 antibody |
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BMP4 antibody |
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| 70R-12408 | Fitzgerald | 100 ug | EUR 403 |
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Description: Rabbit polyclonal BMP4 antibody |
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BMP4 antibody |
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| 70R-12409 | Fitzgerald | 100 ug | EUR 403 |
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Description: Rabbit polyclonal BMP4 antibody |
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BMP4 protein |
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| 30R-1255 | Fitzgerald | 100 ug | EUR 224 |
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Description: Purified recombinant Human BMP4 protein |
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BMP4 protein |
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| 30R-2651 | Fitzgerald | 10 ug | EUR 341 |
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Description: Purified recombinant Human BMP4 protein |
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BMP4 protein |
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| 30R-AB029 | Fitzgerald | 5 ug | EUR 273 |
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Description: Purified recombinant Human BMP4 protein |
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pENTR223- BMP4 |
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| PVT10009 | Lifescience Market | 2 ug | EUR 266 |
pCDNA3.1- BMP4 |
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| PVT10207 | Lifescience Market | 2 ug | EUR 301 |
BCA Reagent, 16ML |
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| C144-16ML | Arbor Assays | 16ML | EUR 163 |
Dissociation Reagent, 1ML |
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| X017-1ML | Arbor Assays | 1ML | EUR 109 |
Dissociation Reagent, 25ML |
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| X017-25ML | Arbor Assays | 25ML | EUR 258 |
Dissociation Reagent, 5ML |
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| X017-5ML | Arbor Assays | 5ML | EUR 122 |
Dissociation Reagent, 1ML |
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| X058-1ML | Arbor Assays | 1ML | EUR 73 |
Dissociation Reagent, 5ML |
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| X058-5ML | Arbor Assays | 5ML | EUR 109 |
Biolipidure-1002-Reagent |
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| Biolipidure-1002-10 | Cusabio | 10mL | EUR 196 |
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Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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HighGene transfection reagent |
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| RM09014 | Abclonal | 1000μl | EUR 270 |
LP4K Transfection Reagent |
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| LP4K | GenTarget | 1.0 ml / vial | EUR 304 |
|
Description: Lipid based transfection reagent for large plasmid and multiple plasmid transfection in both adhesive and suspenstion cell types. |
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n-Heptane Reagent |
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| HC5400 | Bio Basic | 1L | EUR 79 |
Tri-RNA Reagent |
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| FATRR-001 | Favorgen | 100ml | EUR 236 |
Tri-RNA Reagent |
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| FATRR-002 | Favorgen | 50ml | EUR 176 |
Tri-RNA Reagent |
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| FATRR-003 | Favorgen | 450ml | EUR 645 |
DTT (Cleland's reagent) |
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| DB0058 | Bio Basic | 5g | EUR 84.8 |
DTNB (Ellman's Reagent) |
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| DB0113 | Bio Basic | 5g | EUR 97.85 |
Ethyl acetate Reagent |
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| EC4600 | Bio Basic | 1L | EUR 79 |
TissueDigest Reagent, 20X |
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| T101 | Insitus Biotechnologies | 10ml | EUR 210 |
Convoy? Transfection Reagent |
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| 1110-1ml | ACTGene | EUR 341 | |
Griess Reagent Kit |
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| 30100 | Biotium | 1KIT | EUR 149 |
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Description: Minimum order quantity: 1 unit of 1KIT |
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PhosphoBlocker Blocking Reagent |
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| AKR-103 | Cell Biolabs | 1L | EUR 328 |
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Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins. |
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PhosphoBlocker Blocking Reagent |
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| AKR-104 | Cell Biolabs | 4L | EUR 711 |
|
Description: Most commercially available Western blot blockers, such as dry milk or serum, are sufficient to block unreactive sites on the membrane. However, they are not designed to preserve phosphoprotein antigens during blotting. Our PhosphoBLOCKER Blocking Reagent provides superior blocking by maximizing signal-to-noise ratio. The PhosphoBLOCKER reagent particluarly excels with very low levels of endogenous phopsphoproteins. |
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EL Transfection Reagent |
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| 20-abx098880 | Abbexa |
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Mycoplasma Prevention Reagent |
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| 20-abx098886 | Abbexa |
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Girard's reagent T |
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| 20-abx184099 | Abbexa |
|
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FcR blocking Reagent |
|||
| 20-abx290024 | Abbexa |
|
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Detection Reagent A |
|||
| abx296004-120ul | Abbexa | 120 ul | EUR 321 |
Mycoplasma Prevention Reagent |
|||
| 20-abx298005 | Abbexa |
|
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Biolipidure-1002-Reagent |
|||
| Biolipidure-1002-100 | Biolipidure | 100mL | EUR 1223 |
|
Description: The Biolipidure-1002-Reagent is a synthetic amphoteric polymer that can be substituted for BSA in tubidimetric immunoassays. Biolipidure-1002 is an excellent blocker and also enhances assay sensitivity. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-103-Reagent |
|||
| Biolipidure-103-10 | Biolipidure | 10mL | EUR 196 |
|
Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
|||
Biolipidure-103-Reagent |
|||
| Biolipidure-103-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-103-Reagent is a synthetic amphoteric polymer that can be substituted for BSA. It has been shown to enhance signals in rapid tests, western blots, and other similar immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-1201-Reagent |
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| Biolipidure-1201-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-1201-Reagent |
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| Biolipidure-1201-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-1201 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-1301-Reagent |
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| Biolipidure-1301-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-1301-Reagent |
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| Biolipidure-1301-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-1301 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-203-Reagent |
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| Biolipidure-203-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-203-Reagent |
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| Biolipidure-203-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-203 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-203 has been shown to enhance signal strength by improving signal-to-noise in ELISAs, EIAs, and related immunoassays. It also functions as an effective blocker and stabilizer in these assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-206-Reagent |
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| Biolipidure-206-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-206-Reagent |
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| Biolipidure-206-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-206 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-206 enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-405-Reagent |
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| Biolipidure-405-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-405-Reagent |
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| Biolipidure-405-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-405 Reagent is a synthetic anionic polymer that can be used to enhance immunochromatographic assays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-502-Reagent |
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| Biolipidure-502-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-502-Reagent |
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| Biolipidure-502-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-502 Reagent is a synthetic cationic polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-702-Reagent |
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| Biolipidure-702-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-702-Reagent |
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| Biolipidure-702-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-702 Reagent is a synthetic amphoteric polymer. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-802-Reagent |
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| Biolipidure-802-10 | Biolipidure | 10mL | EUR 196 |
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Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Biolipidure-802-Reagent |
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| Biolipidure-802-100 | Biolipidure | 100mL | EUR 1223 |
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Description: The Biolipidure-802 Reagent is a synthetic amphoteric polymer that can be substituted for BSA. Biolipidure-802 generally enhances signal strength, functions as an effective blocker, and stabilizes proteins and antibodies in ELISAs, EIAs, Rapid-test, and related immunoassays. Applications include: Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
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Alcohol, Reagent (70%) |
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| EAS500 | ScyTek Laboratories | 500 ml | EUR 79 |
Alcohol, Reagent (70%) |
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| EAS999 | ScyTek Laboratories | 1000 ml | EUR 101 |
PureFection Transfection Reagent |
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| LV750A-1 | SBI | 1 ml | EUR 359 |
Biotin reagent (HRP) |
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| 65C-CE0202 | Fitzgerald | 5 mg | EUR 244 |
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Description: HRP conjugated biotin labelling reagent |
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BSA (Reagent Grade) |
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| 30-AB79 | Fitzgerald | 1 kg | EUR 1552 |
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Description: Reagent Grade Bovine Serum Albumin (99% pure) |
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BSA (Reagent Grade) |
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| 30-AB81 | Fitzgerald | 200 grams | EUR 476 |
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Description: Reagent Grade Sulphydryl Blocked BSA (99% pure) |
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HAMA blocking reagent |
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| 85R-1001 | Fitzgerald | 1 gram | EUR 1974 |
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Description: HAMA Blocking Reagent for use in immunoassays such as ELISA |
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HAMA blocking reagent |
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| 85R-1001P | Fitzgerald | 1 gram | EUR 2190 |
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Description: HAMA Blocking Reagent for use in immunoassays such as ELISA |
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HAMA blocking reagent |
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| 85R-1003 | Fitzgerald | 1 gram | EUR 1974 |
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Description: HAMA Blocking Reagent for use in immunoassays such as Rapid Tests |
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HAMA blocking reagent |
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| 85R-1014 | Fitzgerald | 50 mg | EUR 192 |
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Description: HAMA blocking reagent for use in assays specific for clinical false positive samples |
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HAMA blocking reagent |
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| 85R-1025 | Fitzgerald | 50 mg | EUR 192 |
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Description: HAMA blocking reagent for use in immunoassays |
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HAMA blocking reagent |
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| 85R-1026 | Fitzgerald | 50 mg | EUR 192 |
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Description: HAMA blocking reagent for use in immunoassays |
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Specimen Preservation Reagent |
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| DA0970 | Daan Gene | 100 test/kit | Ask for price |
Bradford Dye Reagent |
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| 0209R | AthenaES | 100 ml | EUR 131 |
BODIPY-Acetylene Reagent |
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| 2594-1 | Biovision | EUR 207 | |
BODIPY-Acetylene Reagent |
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| 2594-5 | Biovision | EUR 675 | |
ExFect2000 Transfection Reagent |
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| T202-01 | Vazyme | 0.5 ml | EUR 227 |
ExFect2000 Transfection Reagent |
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| T202-02 | Vazyme | 1 ml | EUR 316 |
ExFect2000 Transfection Reagent |
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| T202-03 | Vazyme | 5 ml | EUR 1052 |
Lung Lysate |
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| 1402 | ProSci | 0.1 mg | EUR 191 |
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Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Brain Lysate |
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| 1403 | ProSci | 0.1 mg | EUR 191 |
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Description: Brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Liver Lysate |
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| 1404 | ProSci | 0.1 mg | EUR 191 |
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Description: Liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Kidney Lysate |
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| 1405 | ProSci | 0.1 mg | EUR 191 |
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Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Spleen Lysate |
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| 1406 | ProSci | 0.1 mg | EUR 191 |
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Description: Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Thymus Lysate |
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| 1409 | ProSci | 0.1 mg | EUR 191 |
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Description: Thymus tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Bladder Lysate |
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| 1410 | ProSci | 0.1 mg | EUR 191 |
|
Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Colon Lysate |
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| 1411 | ProSci | 0.1 mg | EUR 191 |
|
Description: Colon tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
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Moreover, decreased methylation ranges have been discovered in the promoters of cardiac genes Myh6, Myh7, Myh7b, Tnnc1, Tnni3, Tnnt2, Nppa, Nppb, mef2c, mef2d, Camta2, Cdkn1A, and Cdkn1C. Of these 13 genes, 6 (Myh6, Tnnc1, Tnni3, Tnnt2, Nppa, Nppb) and 1 (Cdkn1C) had elevated or decreased gene expression, respectively. Altogether, these knowledge present that DNMT1 is essential in embryonic cardiomyocytes by regulating DNA methylation, gene expression, gene splicing, and cell perform.
