Tropilaelaps mercedesae is without doubt one of the most problematic honey bee parasites and has change into extra threatening to the beekeeping business. Tropilaelaps can simply parasitize immature honey bees (larvae and pupae) and have each deadly and sublethal results on the person employee bees. Our examine for the primary time experimentally assessed the consequences of T. mercedesae on olfactory studying, flight means, homing means in addition to transcriptional changes in parasitized grownup honey bees.
T. mercedesae infestation had adverse impacts on olfactory related perform, flight means, and homing charge. The quantity of the mushroom physique considerably elevated in infested honey bees, which can be correlated to the decrease sucrose responsiveness in addition to decrease studying means in the infested bees.
The gene expression concerned in immune programs and carbohydrate transport and metabolism have been considerably completely different between infested bees and non-infested bees. Moreover, genes perform in cell adhesion play an important function in olfactory sensory in honey bees. Our findings present a complete understanding of European honey bees in response to T. mercedesae infestation, and might be used to additional examine the complicated molecular mechanisms in honey bees beneath parasitic stress.
Loss of inner-envelope Ok+/H+ exchangers impairs plastid rRNA maturation and gene expression
The inner-envelope Ok+ EFFLUX ANTIPORTERS (KEA) 1 and 2 are essential for chloroplast improvement, ion homeostasis, and photosynthesis. However, the mechanisms by which changes in ion flux throughout the envelope have an effect on organelle biogenesis remained elusive. Chloroplast improvement requires intricate coordination between the nuclear genome and the plastome. Many mutants compromised in plastid gene expression (PGE) show a virescent phenotype, i.e. delayed greening.
The phenotypic look of Arabidopsis thaliana kea1 kea2 double mutants fulfills this criterion, but a hyperlink to PGE has not been explored. Here, we present {that a} simultaneous lack of KEA1 and KEA2 outcomes in maturation defects of the plastid ribosomal RNAs. This could also be brought on by secondary construction changes of rRNA transcripts and concomitant lowered binding of RNA-processing proteins, which we documented in the presence of skewed ion homeostasis in kea1 kea2.
Consequently, protein synthesis and steady-state ranges of plastome-encoded proteins stay low in mutants. Disturbance in PGE and different indicators of plastid malfunction activate GUN1-dependent retrograde signaling in kea1 kea2, ensuing in a dramatic downregulation of GOLDEN2-LIKE transcription components to halt expression of photosynthesis-associated nuclear-encoded genes (PhANGs). PhANG suppression delays the event of absolutely photosynthesizing kea1 kea2 chloroplasts, in all probability to keep away from progressing photo-oxidative harm. Overall, our outcomes reveal that KEA1/KEA2 perform impacts plastid improvement by way of results on RNA-metabolism and PGE.
Effects of Tris (2-chloroethyl) phosphate (TCEP) on survival, development, histological changes and gene expressions in juvenile yellow catfish Pelteobagrus fulvidraco
Tris (2-chloroethyl) phosphate (TCEP) is an rising aquatic environmental pollutant. In the current examine, juvenile yellow catfish (Pelteobagrus fulvidraco) have been uncovered to environmentally related concentrations of TCEP for 30 days. The outcomes confirmed that TCEP publicity decreased the survival charge (100 μg/L), physique weight (10 and 100 μg/L) and particular development charge (10 and 100 μg/L) of juvenile yellow catfish.
Exposure to TCEP resulted in pronounced damages of gill buildings. Gene transcription evaluation confirmed that the antioxidant capability of the liver and gills was affected; CYP1A1 may contribute to section I metabolism of TCEP in the liver slightly than CYP1B1; TCEP stress may improve the demand of ion transport in fish gill; TCEP may stimulate the immune response and may induce apoptosis by way of a p53-Bax pathway and caspase-dependent pathway in gills. Collectively, these findings present new insights into the poisonous results of TCEP on fish.

Knockdown of DNA methyltransferase 1 reduces DNA methylation and alters expression patterns of cardiac genes in embryonic cardiomyocytes
We beforehand discovered that DNA methyltransferase 3a (DNMT3a) performs an essential function in regulating embryonic cardiomyocyte gene expression, morphology, and perform. In this examine, we investigated the function of essentially the most plentiful DNMT in mammalian cells, DNMT1, in these processes. It is understood that DNMT1 is crucial for embryonic improvement, throughout which it’s concerned in regulating cardiomyocyte DNA methylation and gene expression.
We used siRNA to knockdown DNMT1 expression in major cultures of mouse embryonic cardiomyocytes. Immunofluorescence staining and multi-electrode array have been respectively utilized to judge cardiomyocyte development and electrophysiology. RNA-sequencing (RNA-Seq) and multiplex bisulfite sequencing have been respectively carried out to look at gene expression and promoter methylation. At 72 hours post-transfection, discount of DNMT1 expression decreased the quantity and elevated the scale of embryonic cardiomyocytes.
Beat frequency and the amplitude of area motion potentials have been decreased by DNMT1 siRNA. RNA-Seq evaluation recognized 801 upregulated genes and 494 downregulated genes in the DNMT1 knock-down cells when in comparison with controls. Pathway evaluation of the differentially expressed genes revealed pathways that have been related to cell demise and survival, cell morphology, cardiac perform, and cardiac illness. Alternative splicing evaluation recognized 929 differentially expressed exons, together with 583 up-regulated exons and 308 down-regulated exons.
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
DLR-BMP4-Hu-48T |
DL Develop |
48T |
EUR 510 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
DLR-BMP4-Hu-96T |
DL Develop |
96T |
EUR 657.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
DLR-BMP4-Mu-48T |
DL Develop |
48T |
EUR 522 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
DLR-BMP4-Mu-96T |
DL Develop |
96T |
EUR 673.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
DLR-BMP4-p-48T |
DL Develop |
48T |
EUR 591.6 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
DLR-BMP4-p-96T |
DL Develop |
96T |
EUR 769.2 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Porcine Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
DLR-BMP4-Ra-48T |
DL Develop |
48T |
EUR 544.8 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
DLR-BMP4-Ra-96T |
DL Develop |
96T |
EUR 704.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
DLR-BMP4-Rb-48T |
DL Develop |
48T |
EUR 544.8 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
DLR-BMP4-Rb-96T |
DL Develop |
96T |
EUR 704.4 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rabbit Bone Morphogenetic Protein 4 (BMP4) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 592.8 |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 820.8 |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-c-48Tests |
Reddot Biotech |
48 Tests |
EUR 566.4 |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-c-96Tests |
Reddot Biotech |
96 Tests |
EUR 783.6 |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-Ch-48Tests |
Reddot Biotech |
48 Tests |
EUR 540 |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-Ch-96Tests |
Reddot Biotech |
96 Tests |
EUR 746.4 |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-g-48Tests |
Reddot Biotech |
48 Tests |
EUR 606 |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-g-96Tests |
Reddot Biotech |
96 Tests |
EUR 840 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 501.6 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 690 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 514.8 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 709.2 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 592.8 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 820.8 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 540 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 746.4 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-Rb-48Tests |
Reddot Biotech |
48 Tests |
EUR 540 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RD-BMP4-Rb-96Tests |
Reddot Biotech |
96 Tests |
EUR 746.4 |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-b-48Tests |
Reddot Biotech |
48 Tests |
EUR 619.2 |
Bovine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-b-96Tests |
Reddot Biotech |
96 Tests |
EUR 859.2 |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-c-48Tests |
Reddot Biotech |
48 Tests |
EUR 591.6 |
Canine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-c-96Tests |
Reddot Biotech |
96 Tests |
EUR 819.6 |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-Ch-48Tests |
Reddot Biotech |
48 Tests |
EUR 564 |
Chicken Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-Ch-96Tests |
Reddot Biotech |
96 Tests |
EUR 781.2 |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-g-48Tests |
Reddot Biotech |
48 Tests |
EUR 633.6 |
Goat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-g-96Tests |
Reddot Biotech |
96 Tests |
EUR 879.6 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 523.2 |
Human Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 721.2 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 536.4 |
Mouse Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 741.6 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-p-48Tests |
Reddot Biotech |
48 Tests |
EUR 619.2 |
Porcine Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-p-96Tests |
Reddot Biotech |
96 Tests |
EUR 859.2 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 564 |
Rat Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 781.2 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-Rb-48Tests |
Reddot Biotech |
48 Tests |
EUR 564 |
Rabbit Bone Morphogenetic Protein 4 (BMP4) ELISA Kit |
RDR-BMP4-Rb-96Tests |
Reddot Biotech |
96 Tests |
EUR 781.2 |
Chymase reagent |
30C-CP1129 |
Fitzgerald |
5 units |
EUR 2622 |
Description: Purified native Human Chymase reagent |
Traut's Reagent |
2330-1000 |
Biovision |
|
EUR 418.8 |
BOP reagent |
A7015-100000 |
ApexBio |
100 g |
EUR 240 |
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
BOP reagent |
A7015-25000 |
ApexBio |
25 g |
EUR 135.6 |
Description: A peptide coupling reagent. Can be used in the preparation of phenyl esters of amino acids which have been shown to be valuable as blocked derivatives of amino acids in the field of peptide synthesis. |
Bradford reagent |
BDE641 |
Bio Basic |
100ml |
EUR 73.21 |
|
BMP4 antibody |
70R-12408 |
Fitzgerald |
100 ug |
EUR 483.6 |
Description: Rabbit polyclonal BMP4 antibody |
BMP4 antibody |
70R-12409 |
Fitzgerald |
100 ug |
EUR 483.6 |
Description: Rabbit polyclonal BMP4 antibody |
BMP4 Antibody |
43852-100ul |
SAB |
100ul |
EUR 302.4 |
BMP4 antibody |
70R-16011 |
Fitzgerald |
50 ul |
EUR 522 |
Description: Rabbit polyclonal BMP4 antibody |
BMP4 antibody |
38256-100ul |
SAB |
100ul |
EUR 302.4 |
BMP4 protein |
30R-1255 |
Fitzgerald |
100 ug |
EUR 268.8 |
Description: Purified recombinant Human BMP4 protein |
BMP4 protein |
30R-2651 |
Fitzgerald |
10 ug |
EUR 409.2 |
Description: Purified recombinant Human BMP4 protein |
BMP4 Antibody |
35652-100ul |
SAB |
100ul |
EUR 302.4 |
BMP4 antibody |
20R-2936 |
Fitzgerald |
100 ul |
EUR 471.6 |
Description: Rabbit polyclonal BMP4 antibody |
BMP4 protein |
30R-AB029 |
Fitzgerald |
5 ug |
EUR 327.6 |
Description: Purified recombinant Human BMP4 protein |
BMP4 antibody |
10R-B119a |
Fitzgerald |
100 ug |
EUR 950.4 |
Description: Mouse monoclonal BMP4 antibody |
BMP4 antibody |
10R-B119B |
Fitzgerald |
500 ug |
EUR 327.6 |
Description: Mouse monoclonal BMP4 antibody |
BMP4 antibody |
10R-2148 |
Fitzgerald |
100 ul |
EUR 483.6 |
Description: Mouse monoclonal BMP4 antibody |
BMP4 antibody |
10R-3460 |
Fitzgerald |
100 ul |
EUR 829.2 |
Description: Mouse monoclonal BMP4 antibody |
BMP4 siRNA |
20-abx909174 |
Abbexa |
|
|
|
BMP4 siRNA |
20-abx909175 |
Abbexa |
|
|
|
BMP4 Antibody |
1-CSB-PA763132 |
Cusabio |
|
|
|
Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100 |
BMP4 Antibody |
1-CSB-PA05799A0Rb |
Cusabio |
|
|
|
Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat, Zebrafish. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC; Recommended dilution: WB:1:500-1:5000, IHC:1:100-1:1000 |
BMP4 Antibody |
1-CSB-PA212919 |
Cusabio |
|
|
|
Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:30-1:150 |
BMP4 Antibody |
1-CSB-PA002740GA01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against BMP4. Recognizes BMP4 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB |
anti-BMP4 |
LF-PA0093 |
Abfrontier |
100 ul |
EUR 400.8 |
Description: Rabbit polyclonal to BMP4 |
HEK-293T Telomerase Over-Expressing Cell Pellet |
abx069991-1Pellet |
Abbexa |
1 Pellet |
EUR 477.6 |
|
Esophagus Lysate |
1365 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Esophagus tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Ileum Lysate |
1367 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Ileum tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Rectum Lysate |
1373 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Rectum tissue lysate was prepared by homogenization in homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Skin Lysate |
1376 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Skin tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Thyroid Lysate |
1380 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Thyroid tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Spleen Lysate |
1406 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Bladder Lysate |
1410 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Bladder tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Cerebellum Lysate |
1412 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Cerebellum tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Cerebrum Lysate |
1413 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Cerebrum tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Pancreas Lysate |
1414 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Pancreas tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Stomach Lysate |
1415 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Stomach tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Testis Lysate |
1416 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Testis tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Adrenal Lysate |
1417 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Adrenal tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Skin Lysate |
1419 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Skin tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Eye Lysate |
1420 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Eye tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Trachea Lysate |
1422 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Trachea tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Lung Lysate |
1462 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Liver Lysate |
1464 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Liver tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Kidney Lysate |
1465 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Kidney tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Spleen Lysate |
1466 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
L1210 Lysate |
1284 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: L1210 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The L1210 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
C2C12 Lysate |
1285 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: C2C12 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The C2C12 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
P815 Lysate |
1286 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: P815 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The P815 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
EL4 Lysate |
1287 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: EL4 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The EL4 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Lung Lysate |
1302 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Lung tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Spleen Lysate |
1306 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Spleen tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Placenta Lysate |
1309 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Placenta tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Melanoma Lysate |
20-101 |
ProSci |
0.1 mg |
EUR 632.4 |
Description: Melanoma lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human melanoma tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the melanoma tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The melanoma tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Trachea Lysate |
20-102 |
ProSci |
0.1 mg |
EUR 500.1 |
Description: Human trachea lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human trachea tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the trachea tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The trachea tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Jurkat Lysate |
1205 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Jurkat lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The Jurkat lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
MOLT4 Lysate |
1206 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: MOLT4 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The MOLT4 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
HL60 Lysate |
1209 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: HL60 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The HL60 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
T24 Lysate |
1213 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: T24 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The T24 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
U937 Lysate |
1215 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: U937 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The U937 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
MCF7 Lysate |
1219 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: MCF7 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The MCF7 lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Ramos Lysate |
1225 |
ProSci |
0.1 mg |
EUR 229.2 |
Description: Ramos lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The Ramos lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Kidney Lysate |
21-104 |
ProSci |
0.1 mg |
EUR 342.6 |
Description: Bovine kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Liver Lysate |
21-105 |
ProSci |
0.1 mg |
EUR 342.6 |
Description: Bovine liver tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The bovine liver tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the liver tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The liver tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Heart Lysate |
21-115 |
ProSci |
0.1 mg |
EUR 342.6 |
Description: Guinea Pig heart tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The guinea pig heart tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the heart tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The heart tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Kidney Lysate |
21-116 |
ProSci |
0.1 mg |
EUR 342.6 |
Description: Guinea Pig kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The guinea pig kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Adrenal Lysate |
21-160 |
ProSci |
0.1 mg |
EUR 468.6 |
Description: Monkey (Cynomolgus) adrenal tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) adrenal tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the adrenal tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The adrenal tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Colon Lysate |
21-179 |
ProSci |
0.1 mg |
EUR 342.6 |
Description: Monkey (Cynomolgus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Gallbladder Lysate |
21-188 |
ProSci |
0.1 mg |
EUR 468.6 |
Description: Monkey (Cynomolgus) gallbladder tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) gallbladder tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the gallbladder tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The gallbladder tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Kidney Lysate |
21-190 |
ProSci |
0.1 mg |
EUR 342.6 |
Description: Monkey (Cynomolgus) kidney tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) kidney tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the kidney tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The kidney tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Lung Lysate |
21-194 |
ProSci |
0.1 mg |
EUR 342.6 |
Description: Monkey (Cynomolgus) lung tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) lung tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the lung tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The lung tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Skin Lysate |
21-204 |
ProSci |
0.1 mg |
EUR 468.6 |
Description: Monkey (Cynomolgus) skin tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) skin tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the skin tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The skin tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Spleen Lysate |
21-209 |
ProSci |
0.1 mg |
EUR 342.6 |
Description: Monkey (Cynomolgus) spleen tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Cynomolgus) spleen tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the spleen tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The spleen tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Brain Lysate |
21-272 |
ProSci |
0.1 mg |
EUR 342.6 |
Description: Monkey (Rhesus) brain tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) brain tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the brain tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The brain tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Colon Lysate |
21-288 |
ProSci |
0.1 mg |
EUR 342.6 |
Description: Monkey (Rhesus) colon tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The monkey (Rhesus) colon tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the colon tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The colon tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Moreover, decreased methylation ranges have been discovered in the promoters of cardiac genes Myh6, Myh7, Myh7b, Tnnc1, Tnni3, Tnnt2, Nppa, Nppb, mef2c, mef2d, Camta2, Cdkn1A, and Cdkn1C. Of these 13 genes, 6 (Myh6, Tnnc1, Tnni3, Tnnt2, Nppa, Nppb) and 1 (Cdkn1C) had elevated or decreased gene expression, respectively. Altogether, these knowledge present that DNMT1 is essential in embryonic cardiomyocytes by regulating DNA methylation, gene expression, gene splicing, and cell perform.